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Testing jheh 2 different promoter sequence lengths (a) for transcriptional activity (b) by cloning them into plasmid pCaSpeR-AUG-βgal, transfecting D.Mel2 cells with the recombinant plasmid and assaying 2 × 105 cells for β-galactosidase activity expressed in milliunits (mU). Controls were D.Mel2 cells that were not transfected or transfected with empty plasmid.
