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Testing promoter sequence (225 bp) between pJHEH#3L2 and pJHEH#3L3 (a) for TFs DNA-binding sequences and effects on jheh 3 promoter transcriptional activities, as shown in Figure 7, by testing different promoter lengths and mutating or deleting TF TCF-1 DNA-binding site using primers DB877 and DB876, respectively (b,c).
