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. 2022 Jul 16;12(7):991. doi: 10.3390/biom12070991

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Northern blot analysis of TF Sp1 in D.Mel2 cells that were transfected with promoter pJHEH#3L3 and treated with dsRNA against Sp1. (a) In cells that were treated with dsRNA, the Sp1 transcript is degraded (right lane) compared with untreated cells (left lane). (b) Transcriptional activity of pJHEH#3L2 and pJHEH#3L3, in transfected D.Mel2 cells is not affected, whereas the activity in cells that were treated with dsRNA is similar to cells that were not transfected (NT) or cells that were transfected with an empty plasmid ©.