Table 2.

Primer sequences utilized for qRT-PCR analysis of targeted gene expression.

Target Gene	Primer Sequence (5′–3′)	Accession No./Reference
Occludin	F-CTGTCTATGCTCGTCATCG R-CATTCCCGATCTAATGACGC	NM-031329
JAM	F-GCTCAGCC ATACAGCAAATCC R-GGGAGTCGGGCAAT CATCAG	NM_017232
MUC-2	F-CAGAGTGCATCAGTGGCTGT R-CCCGTCGAAGGTGATGTAGT	XM_039101270.1
Nrf-2	F-GGTTGCCCACATTCCCAAAC R-GGCTGGGAATATCCAGGGCA	NM_031789.2
HO-1	F-CCCAGAGGCTGTGAACTCTG R-AGGCCCAAGAAAAGAGAGCC	NM_012580.2
CD-4	F-AGAAAGGACTGGCCAGAGAC R-CTGAAAGAGAAGCCTCGGCA	NM_031512.2
CD-8	F-ACTCACGGAGTGTGCTGAAG R-CCGCTCTGGCATCATCTTCA	NM_031539.2
TLR-4	F-TCCCACTCGAGGTAGGTGTT R-TTGTTAAGCTTATAAATCATGCGGCCTCAGG	NM_019178.2
β-actin	F-CGCAGTTGGTTGGAGCAAA R-ACAATCAAAGTCCTCAGCCACAT	V01217.1

Junction adhesion molecule (JAM), mucin-2 (MUC-2), Nuclear factor erythroid 2-related factor 2 (Nrf2), Heme oxygenase-1 (HO-1), gene encoding the CD4 membrane glycoprotein of T lymphocytes (CD-4), toll-like receptors (TLR4).