Figure 6.

(A) Schematic illustration of a new GSH-bio-orthogonal reaction that enable to decipher the nanoemitters of cyanine-pyrene dye (1). Resulting in turn-on binary fluorescence signals with a large shift excitation/emission profiles in living systems. (B) GSH-based bio-orthogonal reaction for intracellular application. (a) CLSM images of the nanoemitters 1 (30 μM) in MCF-7 cells (control), LPA (a lipoic acid, a GSH enhancer) pretreated cells (1.8 mM, 24 h, positive) and NEM (N-ethylmalemide a GSH scavenger) treated cells (500 μM, 2 h, negative). (b) Representative line plot of MCF-7 cells and the corresponding fluorescence signal distribution based on the white line in images. (c) The MALDI-TOF spectrum of MCF-7 cell lysates after incubation with the nanoemitters 1 (50 μM) for 3 h (Reproduced with the permission from Ref. [73]).