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. 2022 May 20;9(21):2105806. doi: 10.1002/advs.202105806

Figure 6.

Figure 6

Genome editing on CTCs by the cancer‐targeting delivery system and detection on CXCR4 in genome‐edited CTCs as compared with unedited CTCs. a) The procedure of genome editing, and detection on CXCR4 mRNA and CXCR4. b) CLSM observation on unedited and edited CTCs with CXCR4 mRNA probed by the molecular beacon delivery system. Cell nuclei were stained by DAPI (blue). Scale bar: 15 µm. c) Fluorescence intensity of CXCR4 mRNA probed by the molecular beacon delivery system in unedited and edited CTCs. All CTCs from the patient BC1 are shown in Figure S13, Supporting Information. Fluorescence intensity of each CTC was analyzed by Volocity Demo 6.1.1 software. d) CLSM observation on unedited and edited CTCs with antibody labeled CXCR4 protein. Cell nuclei were stained by DAPI (blue). Scale bar: 15 µm. e) Fluorescence intensity of antibody labeled CXCR4 protein in unedited and edited CTCs from the patient SCLC. Fluorescence intensity of each CTC was analyzed by Volocity Demo 6.1.1 software. Statistical analysis was performed by using Student's t‐test. ****P< 0.0001.