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. 2022 May 20;9(21):2105806. doi: 10.1002/advs.202105806

Figure 7.

Figure 7

Genome editing on CTCs and detection on p53 and p21 in genome‐edited CTCs as compared with unedited CTCs by cancer‐targeting plasmid/molecular beacon co‐delivery systems. a) The procedure of genome editing and p53 mRNA detection. b) CLSM observation on unedited and edited CTCs with p53 mRNA probed by plasmid/molecular beacon co‐delivery systems. Cell nuclei were stained by DAPI (blue). Scale bar: 15 µm. c) Fluorescence intensity of p53 mRNA in unedited and edited CTCs probed by plasmid/molecular beacon co‐delivery systems. All CTCs from the patient BC2 are shown in Figure S14, Supporting Information. Fluorescence intensity of each CTC was analyzed by Volocity Demo 6.1.1 software. d) CLSM observation on unedited and edited CTCs with p21 mRNA probed by plasmid/molecular beacon co‐delivery systems. Cell nuclei were stained by DAPI (blue). Scale bar: 15 µm. e) Fluorescence intensity of p21 mRNA in unedited and edited CTCs probed by plasmid/molecular beacon co‐delivery systems. All CTCs from the patient BC3 are shown in Figure S15, Supporting Information. Fluorescence intensity of each CTC was analyzed by Volocity Demo 6.1.1 software. Statistical analysis was performed by using Student's t‐test. **P< 0.01, ***P< 0.001.