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. 2022 Apr 18;9(21):2105909. doi: 10.1002/advs.202105909

Figure 4.

Figure 4

Generation and characterization of an inflamed joint model in the miniJoint chip. a) The inflamed joint model was created by challenging the SFT via the addition of IL‐1β to the fibrogenic medium (FM). AM, adipogenic medium; OM, osteogenic medium; SM, shared medium. b) Timeline of generating and analyzing the inflamed miniJoint. c) Heat map generated from RNA‐Seq showing the relative expression levels of selected genes in healthy (Ctrl) and inflamed (IL‐1β) miniJoint. AT: adipose tissue; SFT: synovial‐like fibrous tissue; OC‐O, bone component; OC‐C, cartilage component. N = 3 biological replicates. d) TUNEL assay and MMP‐13 immunostaining images confirming the generation of an inflamed SFT. Scale bar = 50 µm. Red arrowheads indicate DNA fragmentation. e) Histological staining and immunostaining of OC‐C showing cartilage degeneration. Scale bar = 50 µm.