Figure 1.

Schematic representation of the MIDAS‐P assembly system for plant expression. The system consists of two entry vectors, pWHITE and pBLUE, for cloning genes of interest and alternate sub‐cloning in the binary destination (expression) vector pMIDAS. The first transcriptional unit is constructed in pWHITE and transferred into pMIDAS using the type IIS restriction enzyme BsaI. A second transcriptional unit in pBLUE can subsequently be transferred into pMIDAS using BsmBI. Further TUs can be added by alternating transfer from pWHITE and pBLUE. The inclusion of lacZα in pMIDAS and pBLUE allows blue/white screening at each stage. The destination vector pMIDAS also has right and left T‐DNA borders for Agrobacterium‐mediated plant transformation. GOI, gene of interest; P, promoter; pA, terminator and polyA signals; SAR, scaffold attachment region; UTR, untranslated region