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. 2022 Mar 16;119(6):1660–1672. doi: 10.1002/bit.28073

Figure 5.

Figure 5

Coexpression of P19 with constructs harboring 3 or 5 TUs. (a) mRNA levels of leaves co‐infiltrated with the pMIDAS construct with 3 TUs for expression of the VRC01 heavy chain (HC), light chain (LC), and Griffithsin (GRFT) with pMIDAS‐P19 were determined by qPCR using the primers listed in Table S2. Data represent the mean of n = 3 biological repeats done in triplicates ± SD. Data were analyzed using Brown–Forsythe and Welch ANOVA tests followed by Tamhane T2 multiple comparison test (***p < 0.001 and ****p < 0.0001). (b) Protein expression levels of VRC01 and GRFT, 6x His‐tagged J3‐VHH (J3His) and DsRed expressed using 3 TU and (c) 5 TU constructs with or without P19 co‐expression. VRC01, GRFT, J3‐VHH, and DsRed expression levels were quantified by ELISA using a human IgGk standard (Sigma‐Aldrich), GRFT standard (gift from Evangelia Vamvaka and Paul Christou), plant‐made J3‐VHH and DsRed standard (gift from Johannes Buyel) respectively. Box plot for expression levels represent the median, minimum, and maximum of three biological repeats. ANOVA, analysis of variance; ELISA, enzyme‐linked immunosorbent assay; mRNA, messenger RNA; TUs, transcription units; SD, standard deviation