TABLE 2.
Purification stage | Total biological activity (AU) | Total proteins (mg) | Sp act (AU/mg) | Increase in sp act (fold) | Yield (%) |
---|---|---|---|---|---|
Supernatant | 21,333 | 4,150 | 5.1 | 1 | 100 |
Sep-pack | 8,960 | 3.4 | 2,635 | 517 | 40 |
RP-HPLC 1 | 2,238 | 0.3 | 6,662 | 1,306 | 10 |
RP-HPLC 2 | 900 | 0.0133 | 67,669 | 13,268 | 4.21 |