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. 2022 Mar 21;41(4):905–917. doi: 10.1002/nau.24908

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© 2022 The Authors. Neurourology and Urodynamics published by Wiley Periodicals LLC

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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(A) Gross appearance of the sheep vaginal tissue (SVT) before and after decellularization in two different solutions showing visible differences in appearance as the decellularized tissue is whiter in color and delicate upon handling with forceps compared to the native SVT. (B) Light microscopy images of primary sheep vaginal epithelial cells and fibroblasts isolated from sheep vagina under ×10 magnification. Epithelial cells showing cobblestone‐like appearance when cultured on irradiated mouse 3T3s (shown with red arrow heads surrounding the colonies). Vaginal fibroblasts showing flat, elongated morphology (scale bar = 100 μm)