Table 1.

Methods used for the decellularization of SVT

Steps	Detergent method	Hypertonic + detergent method	Duration of treatment
1) Primary decellularization solution	0.25% wt/vol sodium deoxycholate + 0.25% vol/vol Triton X‐100 in PBS supplemented with 1% CAS	1 M NaCl in PBS supplemented with 1% CAS	Incubated for overnight on a Platform Rocker STR6 at 40 r/min
2) Epithelium removal	Gentle scraping with sterile forceps	Gentle scraping with sterile forceps	–
3) Washing (twice)	Sterile PBS	Sterile PBS	30 min each
4) Secondary decellularization solution	0.25% wt/vol sodium deoxycholate + 0.5% vol/vol Triton X‐100 in 100 ml PBS supplemented with 1% CAS	0.25% wt/vol sodium deoxycholate + 0.5% vol/vol Triton X‐100 in 100 ml PBS supplemented with 1% CAS	Incubated for 4 days on a Platform Rocker STR6 at 40 REV/min
5) Washing (twice)	Sterile PBS	Sterile PBS	30 min each
6) Storage of decellularized matrices	At 4°C in sterile PBS supplemented with 1% vol/vol P/S	At 4°C in sterile PBS supplemented with 1% vol/vol P/S	Upto 1 month

Abbreviations: CAS, Cambridge Antibiotic Solution; PBS, phosphate‐buffered saline; P/S, 100 IU/ml penicillin, 100 μg/ml streptomycin; SVT, sheep vaginal tissue.