Table 2.

Composition of Green's medium

Component and supplier	Volume and stock solution	Final concentration	Storage
Dulbecco's modified Eagle's medium (GIBCO)	330 ml	66% vol/vol	4°C
Nutrient mixture F12 (Ham's F12) (GIBCO)	108 ml	21.6% vol/vol	4°C
Fetal bovine serum (Advanced Protein Products Ltd.)	50 ml	10% vol/vol	−20°C
Penicillin/streptomycin	5 ml of 10 000 IU/ml penicillin and 10 000 μg/ml streptomycin	100 IU/ml penicillin 100 μg/ml streptomycin	−20°C
Amphotericin B	1.25 ml of 250 μg/ml	0.625 μg/ml	−20°C
Adenine	2 ml of 6.25 μg/ml	0.025 μg/ml	−20°C
Insulin	2.5 ml of 1 mg/ml	5 μg/ml	4°C
3,3,5‐triiodothyronine/apotransferrin	0.5 ml of 1.36 μg/ml T3 and 5 mg/ml apotransferrin	1.36 ng/ml T3 and 5 μg/ml apotransferrin	−20°C
Hydrocortisone	80 μl of 2.5 mg/ml	4 μg/ml	4°C
Epidermal growth factor	25 μl of 100 μg/ml	5 ng/ml	−20°C
Cholera toxin	500 μl of 8.47 μg/ml	8.47 ng/ml	4°C

Note: All components are from Invitrogen unless otherwise indicated.