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Composition of supplemented Dulbecco's modified Eagle's medium (DMEM)
| Component and supplier | Volume and stock solution | Final concentration | Storage |
|---|---|---|---|
| DMEM (GIBCO) | 445 ml | 89% vol/vol | 4°C |
| Fetal bovine serum (Advanced Protein Products Ltd.) | 50 ml | 10% vol/vol | −20°C |
| Penicillin/streptomycin (Invitrogen) | 5 ml of 10 000 IU/ml penicillin and 10 000 μg/ml streptomycin | 100 IU/ml penicillin | −20°C |
