Figure 20.

Kinetic requirements of sensors for spatiotemporal imaging. a) A stochastic‐kinetic simulation allows the binding states (blue) to be predicted and consequently the fluorescence traces of single sensors in response to a changing concentration profile (red), for example, release of molecules from a cell. b) Relative intensity response of a single sensor over time based on the concentration profile shown in (a) and the assumption of 10 binding sites. c) Spatial and temporal resolution phase diagram as a function of rate constants. The red regions show combinations of rate constants that allow the detection of the biological events (here, release of molecules from a cell). Reprinted from Ref. [180] with permission. Copyright 2017 American Chemical Society.