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. 2022 Mar 30;61(22):e202200994. doi: 10.1002/anie.202200994

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© 2022 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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In vitro biosynthesis of the FeGP cofactor. a) Gas requirements. Addition of formate is indicated above the bars. (−) no precursor was added. b) Kinetics of biosynthesis from 3. Assay with full component (+) and with no precursor (−). c) Inhibition of activity obtained from 3 by TosMIC. d) Activity obtained from the precursors by complementation with HcgB and/or HcgC. Compounds 1, 2, 3 or no precursor (−) were added to the assay (abscissa). The Hcg enzymes added are indicated above the bar.