Figure 3.

Requirement and incorporation of substances in in vitro biosynthesis. a) Dependency on the substances. (+) Assay with full component of the standard assay. Omission of the substances and addition of potential inhibitors in the absence of SAM, methionine (Met), S‐adenosyl‐L‐homocysteine (SAH) and 5′‐deoxyadenosine (dAdo). b) Effect of CO concentrations in N₂. Partial pressure of CO in the CO/N₂ mixture in the 1.1 bar gas phase is shown. ¹³C isotope enrichment of the FeGP cofactor (c) and its decomposed product 3 (d) from [¹³C]‐CO as indicated by the fractional abundance of the corresponding ¹³C isotope isomers. The fractional abundance corresponds to the experimentally observed isotope distribution, corrected for the natural isotope abundance contribution using IsoCor (Supporting Information). ^[13]