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. 2022 Mar 19;61(20):e202116403. doi: 10.1002/anie.202116403

Figure 1.

Figure 1

The T193A mutation within the CTD of DNAJB6b does not significantly affect the CTD architecture. A) Chemical shift differences for backbone Cα, Cβ, N, HN atoms of T193A relative to WT CTD (Δδω=δωWT‐CTD–δωT193A). The T193A site is shown as a red bar. Note that R207 is hydrogen bonded to S192. B) Strips of the 600 MHz non‐uniformly sampled 3D HHC NOE‐HSQC spectrum (mixing time 200 ms) collected on a 500 μΜ U‐[13C, 15N]‐labeled T193A sample at 298 K. C) Correlation between 3 J HN‐Hα couplings of T193A and WT CTDs measured using the ARTSY approach [12] (left). Cartoon representation (right) of the refined T193A structure, with residues for which NOEs are observed in (B) shown in green and A193 shown in red. Residues for which coupling constants differ between the two constructs (perhaps due to different amplitude of ms motions [9b] ) are shown in pink. The correlation coefficient and RMSD exclude the residues in pink that display ms dynamics.