Delayed activation of heading regulatory genes in a higher temperature environment.
(a) Temperatures in the plastic shed and greenhouse. Chiifu in the shed was named CFP, while Chiifu in the greenhouse was named CFG.
(b) Delayed heading process of CFG as shown by photos.
(c) Histograms showing the variation in expression peak of genes in CFP and CFG. Upper panel shows the frequency of gene expression peaks locating in the transition stage of CFP, while the bottom panel shows their corresponding expression peaks in CFG. The time point with the largest number of genes shifted from T6 in CFP to T7 in CFG, indicating a delay in expression peak. (d) The vast majority of transition‐activated Chiifu genes as identified above were delayed in CFG. According to the dendrogram, these genes were divided into five clades. (e) Expression patterns of genes in five clades as identified in (d). Transition stage is marked by bold fonts on an orange background. TPM, transcript per million. [Colour figure can be viewed at wileyonlinelibrary.com]