Table 1.

List of primers used in real-time PCR.

RNA template	Primer sequences (forward:5′ and reverse:3′)	Annealing temperature (°C)
GAPDH	5′-TGGTATCGTGGAAGGACTCATGAC 3′-ATGCCAGTGAGCTTCCCGTTCAGC	60
IL-1β	5′-GTGGCAATGAGGATGACTTGTT 3′-TGGTGGTCGGAGATTCGTAG	60
IL-6	5′-TAGTGAGGAACAAGCCAGAG 3′-GCGCAGAATGAGATGAGTTG	60
IL-8	5′-CCAAACCTTTCCACCC 3′-ACTTCTCCACAACCCT	60
MCP-1	5′-GAAGCTCGCACTCTCGCCT′ 3′-GAGTGTTCAAGTCTTCGGA′	60
TNF-α	5′-AGCCCATGTTGTAGCAAACC 3′-ACCTGGGAGTAGATGAGGTA	60
MMP-1	5′-TGGACCTGGAGGAAATCTTG 3′-CCGCAACACGATGTAAGTTG	60
MMP-10	5′-GCCAGTCCATGGAGCAAGGCT′ 3′-TCGCCTAGCAATGTAACCAGCTGT	60
ADAMTS4	5′-CTGCCTACAACCACCG 3′-GCAACCAGAACCGTCC	60
ADAMTS5	5′-GCACTTCAGCCACCATCAC 3′-AGGCGAGCACAGACATCC	60
MMP-13	5′-TCACGATGGCATTGCT 3′-GCCGGTGTAGGTGTAGA	60
COX-2	5′-CAGCACTTCACGCATCAGTTT 3′-GCGCAGTTTACGCTGTCTA	60
INOS	5′-ACATTGATCAGAAGCTGTCCCAC 3′-AAAGGCTGTGAGTCCTGCAC	60
IL-10	5′-CTTTAAGGGTTACCTGGGTTG 5′-CTTGATGTCTGGGTCTTGG	60
VEGF	5′-TGATGATTCTGCCCTCCTC 3′-GCCTTGCCTTGCTGCTC	60