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. 2022 Mar 28;35(8):1022–1033. doi: 10.1038/s41379-022-01058-y

Fig. 2. Human xenografted CTCs can be detected in mouse blood by identification of the expected fusion transcript using size selection and ddPCR.

Fig. 2

A Cartoon of the size enrichment of circulating tumor cells using either the Celsee chip or ScreenCell filter. Large tumor cells are not able pass through the 6 µm pores present in either the microfluidic chip or filter. B Relative bioluminescence (mean and standard error of the mean) of 23 mice at day 14, 21, and 28 after cell line IV injection of A673 (left panel) and TC71 (right panel) showing very high tumor burden at day 28 in Ewing sarcoma xenograft mouse models. C Blood samples collected from mice bearing high burden of metastatic xenografted Ewing sarcoma cells were size enriched and subjected to EWSR1-FLI1-specific ddPCR. Absolute expressions of EWSR1-FLI1 transcript is represented, showing that all 23 samples had detectable CTCs. D Multiplex transcript detection by ddPCR. The EWSR1-FLI1 fusion was also detected in four of these same blood samples using the multiplexed ddPCR primer and probe set described in Fig. 1.