Extended Data Fig. 3. Prolonged breastfeeding reduces lipid droplets and increases UCP1 in adipose tissue.
Prolonged suckling activates browning of WAT. a–d, Effects of delayed weaning on quantification of lipid droplet cross-sectional area in SAT (a); quantification of immunolabeling for UCP1 in SAT (b); SAT protein levels of PGC1α and UCP1 (c); SAT protein levels of pHSL, HSL and ratio pHSL/HSL (d). Protein data were expressed in relation (%) to control (SW-CD) animals. β-actin was used to normalize protein levels. Dividing lines indicate splicing within the same gel. Values are represented as means ± SEM, n per group indicated in each figure. Exact P values are shown. Statistical differences according to a One-way analysis of variance ANOVA (normal data and homogeneity of variances) followed by Tukey’s post hoc multiple comparison test (a and b) or to a two-sided Mann-Whitney U-test (non-normal data and non-homogeneous variance) (c and d).