Extended Data Fig. 4. Prolonged breastfeeding reduces liver steatosis and stimulates FGF21 levels.
Prolonged suckling alleviates HFD-induced steatosis. a–c, Effects of delayed weaning on oil red area in the liver (a); liver protein levels of FGF21 (b); plasma FGF21 levels (c). Protein data were expressed in relation (%) to control (SW-CD) animals. β-actin was used to normalize protein levels. Dividing lines indicate splicing within the same gel. Values are represented as means ± SEM, n per group indicated in each figure. Exact P values are shown. Statistical differences according to a One-way analysis of variance ANOVA (normal data and homogeneity of variances) followed by Tukey’s post hoc multiple comparison test (c) or to a two-sided Mann-Whitney U-test (non-normal data and non-homogeneous variance) (a and b).