Extended Data Fig. 7. FGF21 increases protein levels of D2R in the LHA/ZI.
a, Delayed weaning effects on LHA/ZI protein levels of D2R and Orexin levels in rats fed HFD. b–d, Effect of exposure to cold in mice lacking FGF21 in the liver (Albcre) on infrared thermal images and quantification of BAT interscapular temperature (b); BAT protein levels of UCP1 (c); LHA/ZI protein levels of D2R (d). Protein data were expressed in relation (%) to control (SW) animals. β-actin was used to normalize protein levels. Dividing lines indicate splicing within the same gel. Values are represented as means ± SEM, n per group indicated in each figure. Exact P values are shown. Statistical differences according to a two-sided Student’s t-test (normal data) (b), a two-sided Mann-Whitney U-test (non-normal data and non-homogeneous variance) (a, c and d).