Fig. 2. Prolonged suckling (delay weaning) increases interscapular temperature in both thermoneutral and cold exposureconditions.
a–f, Delayed-weaning effects in rats fed an HFD under thermoneutral conditions on body weight (a); cumulative food intake (b); body temperature (c); infrared thermal images and quantification of BAT interscapular temperature (d); energy expenditure (e) and locomotor activity (f). g,h, Delayed-weaning effects in rats fed an HFD and exposed to cold (4 °C) for 6 h on body temperature (g) and infrared thermal images and quantification of BAT interscapular temperature at 4 °C (h). Values are represented as means ± s.e.m., n per group. Exact P values are shown. Statistical differences were determined by a two-sided Student’s t-test (normal data; c–h), a two-sided Mann–Whitney U test (non-normal data and non-homogeneous variance; a and b), or an ANCOVA with body weight as a covariate (e).