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. 2022 Jul 25;4(7):901–917. doi: 10.1038/s42255-022-00602-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, Timeline of the experimental protocol. b–m, Effect of infection with adenoviral particles encoding shFgf21 in the tail vein of 12-week-old rats fed an HFD after prolonged suckling on liver protein levels of FGF21 (b); plasma FGF21 levels (c); body weight (d); fat mass (e); cumulative food intake (f); infrared thermal images and quantification of BAT interscapular temperature (g); BAT protein levels of PGC1α, UCP1 and FGF21 (h); quantification of immunolabelling for UCP1 in BAT (i); Oil Red area in the liver (j); plasma triglycerides (k); LHA/ZI protein levels of FGF21 (l); and LHA/ZI protein levels of D2R (m). Protein data were expressed as percentages in relation to control (SW-HFD shLuciferase) animals. β-actin was used to normalize protein levels. Dividing lines indicate splicing within the same gel. Values are represented as means ± s.e.m., n per group. Exact P values are shown. Statistical differences were determined by one-way ANOVA (normal data and homogeneity of variances) followed by Tukey’s post hoc multiple-comparison test (c–e, g and i–k) or a two-sided Mann–Whitney U test (non-normal data and non-homogeneous variance; b, f, h, l and m).

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