Fig. 7. D2R signalling in LHA/ZI GABA neurons is required for FGF21 action.
a, Photomicrographs showing the colocalization of GFP and FGFR1 in the LHA/ZI of D2r-Cre mice. b–e, Effect of injecting an adenoviral vector encoding a scrambled RNA (AAV-EF1A-EGFP-floxed) or an shRNA against Fgfr1 (AAV8-EGFP-shFgfr1-floxed) in a Cre-dependent manner, followed by ICV injection of vehicle or FGF21 in D2r-Cre mice on body weight change (b); cumulative food intake at 24 h and 8 h (c); infrared thermal images and quantification of BAT interscapular temperature (n = 11–13; d); and BAT protein levels of PGC1α and UCP1 (e). f, Photomicrographs showing the colocalization of GFP and Vgat in the LHA/ZI of D2r-Cre mice. g–j, Effect of injecting an adenoviral vector encoding a scrambled RNA (Ad-hSyn-DIO-EGFP) or an shRNA against D2r (Ad-hSyn-DIO-shD2r-EGFP) in a Cre-dependent manner, followed by ICV injection of vehicle or FGF21 in Vgat-ires-Cre mice on body weight change (g), cumulative food intake (h); infrared thermal images and quantification of BAT interscapular temperature (i); and BAT protein levels of PGC1α and UCP1 (j). Protein data were expressed as percentages in relation to control (GFP vehicle) animals. β-actin was used to normalize protein levels. Dividing lines indicate splicing within the same gel. Values are represented as means ± s.e.m., n per group. Exact P values are shown. Statistical differences were determined by a two-sided Student’s t-test (normal data; b–d, g and h), or a two-sided Mann–Whitney U test (non-normal data and non-homogeneous variance; e, i and j).