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. 2022 Jul 25;11(7):e12248. doi: 10.1002/jev2.12248

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© 2022 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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ABD‐engineered EVs bind to MSA in vivo after injection. (A) Schematic illustration of the animal experimental method. Plasma was harvested 10 min after I.V injection. (B) The binding assay for the injected ABD‐EVs with MSA after injection. FITC‐labelled MSA antibody was used to incubate with the plasma and SEC was performed to get different fractions. (C) Nluc luciferase activity of the injected EVs. (D) Single vesicles bound to MSA after injection detected by widefield imaging. AF647‐labelled CD9/CD63/CD81 antibodies cocktail was exploited to detect EVs whereas FITC‐labelled MSA antibody was used to confirm the co‐localization of EVs with MSA