FIG. 1.

Organization of the region of the S. coelicolor M145 chromosome containing the accB and accE genes. (A) Genetic and physical map of the 6.2-kb insert in pRM08. The secondary structure downstream of accE may represent a factor-independent transcriptional terminator. Probes 1 and 2 were generated by PCR using the oligonucleotides accBup-accBdown and accBEup-accBEdown, respectively, uniquely labeled at the 5′ end (∗) and were used in transcriptional analysis of the accBE operon. (B) Map of the DNA fragments cloned in pET22b that were used for expression of accB and/or accE in E. coli. Only relevant restriction sites are shown: B, BamHI; Bc, BclI; E, EcoRI; K, KpnI; Nd, NdeI; N, NotI; S, SpHI.