FIG. 5.

Growth phase-dependent expression and transcription start site of the accBE operon. (A) S1 nuclease mapping of accB, actII-ORF4, and hrdB, using RNA isolated from a liquid-grown culture of S. coelicolor M145. Exp, Trans, and Stat indicate the exponential, transition, and stationary phases of growth, respectively. (B) The nucleotide sequence of both strands of the accB promoter region is shown. The arrow indicates the most likely transcription start point for the accBE promoter, as determined by S1 nuclease mapping. Potential −10 and −35 regions for accBEp are underlined. (C) S1 nuclease mapping of the accB-accE intergenic region using a 563-nt probe. FLP, full-length protection of the probe reflecting transcription across the intergenic region.