TABLE 2.

Heterologous expression of ACCase components in cell extracts of E. coli and in vitro reconstitution of enzyme activity

E. coli straina	Protein(s) induced by IPTG	Protein expression (mU mg of protein−1) in cell extractsb
		Acetyl-CoA carboxylase	PCCase
RG7c	AccA1	<0.02e	<0.02e
RG11c	AccA2	<0.02e	<0.02e
RG8	AccB, AccE	<0.02e	<0.02e
RG11-RG8d	AccA2-AccB, AccE	7.53 ± 0.18	9.10 ± 0.20
RG7-RG8d	AccA1-AccB, AccE	1.85 ± 0.12	2.25 ± 0.13

^aAll the RG strains are derived from E. coli BL21(DE3), except RG7, which derives from DH5α. 

^bResults are the means of three determinations ± standard errors. 

^cContains plasmid pBA11 that expresses BirA constitutively. 

^dMix of equal amounts of proteins from cell extracts of each of the strains indicated. 

^eThe amount of ¹⁴C fixed into acid-stable products was not significantly higher than background levels (10 cpm, equivalent to 0.02 mU).