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. 2022 Jul 19;40(3):111126. doi: 10.1016/j.celrep.2022.111126

Figure 2.

Figure 2

Longitudinal evolution of NK cell responses correlates with trajectory of intact HIV-1 proviruses

(A) Linear discriminant analysis of the phenotypic profile of NK cells responses at indicated time points in early-treated infants. NK cells were phenotypically characterized using flow cytometry with 9 distinct surface markers.

(B) Representative flow cytometry pseudocolor plot highlighting subclassification of NK cell subsets stratified according to CD16 and CD56 expression.

(C) Pseudocolor plots indicating expression of CD57 and NKG2A in indicated NK cell subsets at week 0 (immediate after birth) and at 72/84 weeks after birth.

(D and E) Longitudinal evolution of CD57-expressing (D) and NKG2A-expressing (E) NK cell subsets in early-treated infants. Data from weeks 0 (n = 11), 12/24 (n = 12), and 72/84 (n = 18) are shown. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001; Kruskal Wallis test with post-hoc Dunn’s test.

(F and G) Correlation between proportional changes of indicated NK cell subsets (between weeks 0 and 72/84) and corresponding changes in intact HIV-1 proviruses. Spearman correlation coefficient is indicated.