REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti-LC3B	Sigma Aldrich	CST: 2775; RRID: AB_796155
anti-LC3B	Novus Biological	NB100-2220; RRID: AB_10003146
anti-GAPDH	Cell Signaling	CST: 2118; RRID: AB_561053
anti-GAPDH	Sigma Aldrich	G8795; RRID: AB_1078991
anti-TUBB	Sigma Aldrich	T5201; RRID: AB_609915
anti-ACTN	Sigma Aldrich	A1978; RRID: AB_476692
anti-p62/SQSTM1	Sigma Aldrich	P0067; RRID: AB_1841064
anti- βIII-Tub	Thermo Fisher Scientific	MA1-118; RRID: AB_2536829
anti-ACC	Cell Signaling	CST: 3662; RRID: AB_2219400
anti-p-ACC	Cell Signaling	CST: 11818; RRID: AB_2687505
anti-ULK1	Cell Signaling	CST: 8054; RRID: AB_11178668
anti-p-ULKser317	Cell Signaling	CST: 12753; RRID: AB_2687883
anti-AMPK	Cell Signaling	CST: 2352; RRID: AB_330331
anti-p-AMPK	Cell Signaling	CST: 2531; RRID: AB_330330
anti-4EBP1	Thermo Fisher Scientific	AHO1382; RRID: AB_2536336
anti-p-4EBP1	Thermo Fisher Scientific	700238; RRID: AB_2532302
anti-PARP	Cell Signaling	CST: 9542; RRID: AB_2160739
anti-CASP3	Cell Signaling	CST: 9662; RRID: AB_331439
anti-PGC1 α	Thermo Fisher Scientific	PA5-38021; RRID: AB_2554625
anti-COXIV	Thermo Fisher Scientific	20E8C12; RRID: AB_2535839
anti-TFAM	Abcam	ab47517; RRID: AB_945799
anti-NEUN	Millipore	MAB377; RRID: AB_2298772
anti-MAP2	Cell Signaling	CST: 4542S; RRID: AB_10693782
anti- βIII-Tub	GenScript	A01627; RRID: AB_2622164
Chemicals, peptides, and recombinant proteins
Clozapine	Sigma Aldrich	C6305
Chloroquine	Sigma Aldrich	C6628
3-methyladenine	Sigma Aldrich	M9281
bafilomycin A1	Sigma Aldrich	B1793
Idebenone	Sigma Aldrich	I5659
Lipofectamine reagent	Thermo Fisher Scientific	15338100
MitoTracker Green FM	Thermo Fisher Scientific	M7514
LysoTracker Red DND-99	Thermo Fisher Scientific	L7528
MitoSOX Red	Thermo Fisher Scientific	M36008
Tetramethylrhodamine, Methyl Ester, Perchlorate (TMRM)	Thermo Fisher Scientific	T-668
Critical commercial assays
ELISA KIT ATG5	My Biosource	MS7209535
ELISA KIT Parkin	My Biosource	MBS732278
ELISA KIT Optineurin	My Biosource	MBS2704623
ELISA KIT ATG7	My Biosource	MBS062423
NucleoSpin Tissue	Macherey-Nagel	REF 740952.50
Experimental models: Cell lines
Detailed information of cell used in this Research Article are reported in Table S1 and in STAR Methods at the section experimental model and subject details	N/A	N/A
Recombinant DNA
GFP-LC3	(Patergnani et al., 2013)	N/A
mCherry-GFP-LC3	(Patergnani et al., 2013)	N/A
YFP-Parkin	Addgene	Plasmid #23955; RRID: Addgene_23955
mitochondrial (mt)-Cherry	Addgene	Plasmid #55102; RRID: Addgene_55102
pTRE-tight-MITO TIMER	Addgene	plasmid # 50547; RRID: Addgene_50547
Software and algorithms
Graphpad Prism v9.3.1	GraphPad	RRID: SCR_002798
FiJi (ImageJ)	https://imagej.net/software/fiji/	RRID: SCR_002285
EndNoteX9	EndNote	RRID: SCR_014001
Biorender	https://biorender.com/	RRID: SCR_018361