Figure 6.

GCase activity level in control and Gaucher fibroblasts cultured in the presence of 25 mM MES or HEPES. Fibroblast obtained from healthy individuals and Gaucher patients with known GBA genotype were cultured as indicated and GCase activity in cell lysates was determined. Values expressed a mean ± SD; measurements performed in triplicate. Significance (paired t‐test, asterisks) indicates the effect of buffer on control and GD‐patient cells, respectively; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. Hash (#) indicates cell lines derived from patients harboring the same mutation. GD, Gaucher disease; HEPES, 4‐(2‐hydroxyethyl)‐1‐piperazineethanesulfonic acid; MES, 2‐(N‐morpholino)ethanesulfonic acid