Table 1.

Glucosylsphingosine (GlcSph) content of fibroblasts cultured in the absence or presence of 25 mM HEPES

Fibroblast line	No HEPES	+25 mM HEPES (pH 7.4)
	GlcSph (pmol/mg total protein of cell lysate)
Control wt GBA	<0.3	<0.3
Control wt GBA	<0.3	<0.3
N370S/L444P GBA	1.3 ± 0.4	4.2 ± 1.2
N370S/L444P GBA	0.9 ± 0.3	3.3 ± 0.6
N370S/N370S GBA	0.7 ± 0.3	1.4 ± 0.5
L444P/L444P GBA	2.5 ± 0.4	2.4 ± 0.8

Note: Values expressed as mean ± SD on three independent cell cultures and triplicate measurements.

Abbreviation: HEPES, 4‐(2‐hydroxyethyl)‐1‐piperazineethanesulfonic acid.