Table 1.

Molecular characteristics of RNF43 frameshift mutant CRC organoids.

	Organoid lines	C14	C24	C45
Genetic/epigenetic status	RNF43 mutation	p.Pro370fs	p.Pro370fs	p.Arg225fs
		p.Gly659fs	p.Gly659fs	p.Pro370fs
	BRAF mutation	p.Val600Glu	p.Val600Glu	p.Val600Glu
	CIMP	High	High	High
	MSI	High	High	High
Organoid growth	Wnt ligand dependency	+	+	+
	Exogenous Wnt ligand	No need*	No need*	Required
	R‐spondin dependency	+	+	+
	Exogenous R‐spondin	Required	Required	Required

^*C14 and C24 cells utilize endogenously expressed Wnt ligands.