TABLE 3.

Purification of 2,5-diketo-l-gulonic acid reductases^a

Form	Step	Amt (U)	Sp act (U/mg)	Purification (fold)	Recovery (%)
C	Extract	97,500	2.5	1	100
	Matrix Red agarose	59,900	20.4	8.0	61.4
	MonoQ	56,920	60.2	23.7	58.4
D	Extract	116,400	0.36	1	100
	Matrix Red agarose	68,928	2.1	5.8	59.2
	MonoQ I	62,230	16.9	46.6	53.5
	MonoQ II	49,788	20.5	56.9	42.8

^aEnzymes were purified as described in Materials and Methods.