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Comparison of kinetic parameters with NADH as cofactorb
| Form | Parameters
|
||||
|---|---|---|---|---|---|
| Km for NADH (μM) | kcat (s−1) | kcat/Km for NADH (mM−1 s−1) | Km for DKGa (μM) | Km for Pi (mM) | |
| C | 1,800 | 1.6 | 0.9 | 2,260 | 16.8 |
| D | 3,900 | 12.2 | 3.1 | 1,150 | 10.6 |
DKG, 2,5-diketo-d-gluconic acid.
Values for 2,5-diketo-d-gluconic acid were determined in the presence of 0.15 mM NADH, and values for NADH were determined in the presence of 1 mM 2,5-diketo-d-gluconic acid.
