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. 2022 Mar 29;55(7):1742–1755. doi: 10.1111/ejn.15650

FIGURE 3.

FIGURE 3

mir‐32‐5p target genes are regulated after forced abstinence and correlate with mir‐32‐5p expression levels. miRNA expression in yoked saline and morphine animals after 1 day (D) (a) or 30D (b) of forced abstinence with no relapse test. Displayed are fold change values relative to saline animals. Animals that self‐administered high amounts of morphine (35 + infusions per day) are indicated with red circles or low amounts of morphine (<35 infusions per day) are indicated with blue circles. (c, d) mRNA expression of predicted mir‐32‐5p target genes Btg2, Cldn11, Dcaf6, and Dusp5 in animals that received yoked saline or morphine SA and underwent 1D (c) or 30D (d) forced abstinence with no relapse test. (f, g) Correlation analyses of calculated ΔΔCt mir‐32‐5p expression in the NAc with ΔΔCt levels of the target genes Btg2 (e), Cldn11 (f), and Dcaf6 (g) in animals that underwent 30D of forced abstinence from morphine SA. t indicates trend for statistical significance 0.05 < p < 0.1. *p < 0.05. Error ± standard error of the mean (SEM)