Figure 2.

Recombinant protein production driven by the ogt104167 promoter. The figure shows Coomassie blue‐stained sodium dodecyl sulfate polyacrylamide gel electrophoresis gels and western blots (below) that detail the expression of (a, b) recombinant 6His‐GFP, (c) hGH‐6His, and (d) anti‐IL‐1β‐6His scFv in Escherichia coli K‐12 JCB387N11 (ΔnarG) cells grown in minimal salts medium after 3 h induction by the addition of 20 mM sodium nitrate (+) at an OD₆₀₀ of 0.3–0.5. The DNA encoding each target was cloned into pET expression vectors carrying the ogt104167 promoter fragment. Empty vector controls (EV) are included, where indicated.