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. 2022 Mar 6;119(6):1614–1623. doi: 10.1002/bit.28071

Figure 4.

Figure 4

Expression of 6His‐GFP using the narG CC(−40.5) promoter. The figure shows Coomassie blue‐stained sodium dodecyl sulfate polyacrylamide gel electrophoresis gels and western blots (below) of JCB387N11 (ΔnarG) cells expressing 6His‐GFP, using the narG CC(−40.5) promoter. The DNA encoding 6His‐GFP was cloned into pET expression vectors carrying the narG CC(−40.5) promoter fragment. Cells were grown in minimal salts media and RPP was initiated for 3 h (a, c) by the addition of 20 mM sodium nitrate or (b) by a range of sodium nitrate concentrations. In (c), cultures were supplemented with 0.4% glucose, where indicated. Empty vector controls (EV) were included.