Figure 5.

Analysis of 6His‐GFP expression from the narG CC(−40.5) promoter in E. coli JCB387N11 cells at 30 or 37°C. The figure shows the (a) growth, (b) mean GFP fluorescence measured by flow cytometry, (c) productivity (green fluorescence multiplied by OD₆₀₀), and (d) percentage dead cells of 50 ml JCB387N11 (ΔnarG) cultures expressing 6His‐GFP, using the narG CC(−40.5) promoter. Cells were grown in minimal salts media at either 30°C (blue) or 37°C (orange) for 25 h. Recombinant protein production was initiated by the addition of sodium nitrate to a final concentration of 20 mM, as cells reached an OD₆₀₀ of ~0.6. Values represent the average values from duplicate flasks and error bars represent ±SD. (e) Flow cytometry analysis of green fluorescence from cells expressing 6His‐GFP grown and induced at either 30°C (blue) or 37°C (orange). Data are plotted as histograms showing the number of cells with different green fluorescence (FL1‐A) values.