TABLE 4.
Phylogenetic assignment and theoretical T-RF sizes of 51 16S rRNA gene sequences obtained from the clay fraction of the AM treatment as well as the location in soil of corresponding T-RFs in T-RFLP profiles
| Phylogenetic assignment | Theoretical T-RF size (bp) | Size of corresponding T-RF in T-RFLP (bp) | Location in soil |
|---|---|---|---|
| Holophaga/Acidobacterium | 37 | 39 | All fractions (may also derive from gram-positives and Proteobacteria) |
| 76a | 76 | Silt and clay (may also derive from gram-positives) | |
| 92 | 92 | Silt and clay | |
| 96a | 96 | Silt and clay | |
| 206 | 205 | All fractions (may also derive from Proteobacteria) | |
| 214 | 216 | Silt and clay | |
| 220 | 220 | Silt and clay | |
| 222 | 223 | Mainly silt and clay (may also derive from Prosthecobacter) | |
| 233a | 233 | Clay | |
| 234 | 236 | Silt and clay | |
| 235 | 236 | Silt and clay (may also derive from Prosthecobacter or Flexibacter/Cytophaga/Bacteroides) | |
| 242 | 242 | Clay | |
| Verrucomicrobiales (Prosthecobacter) | 100 | 101 | Silt and clay |
| 208 | 208 | All fractions | |
| 222 | 228 | Mainly silt and clay (may also derive from Holophaga/ Acidobacterium) | |
| 225b | |||
| 236 | 236 | Silt and clay (may also derive from Holophaga/Acidobacterium) | |
| 237 | 236 | Silt and clay | |
| Gram-positives | |||
| High G+C | 37 | 39 | All fractions (may also derive from Holophaga/Acidobacterium or Proteobacteria) |
| 38 | 39 | All fractions (may also derive from Holophaga/Acidobacterium or Proteobacteria) | |
| 63 | 62 | Mainly silt and clay | |
| 76a | 76 | Silt and clay (may also derive from Holophaga/Acidobacterium) | |
| 132c | |||
| 210a | |||
| 229 | 228 | All fractions | |
| Low G+C | 88a | ||
| 231 | 231 | All fractions | |
| 232 | 231 | All fractions | |
| 240 | 242 | Clay | |
| 284 | 284 | Clay | |
| 290d | 290 | Mainly sand | |
| Proteobacteria | |||
| α | 39 | 39 | All fractions (may also derive from Holophaga/Acidobacterium or gram-positives) |
| 64 | 66 | All fractions | |
| 83 | 81 | All fractions | |
| 195 | 194 | All fractions | |
| γ | 39 | 39 | All fractions (may also derive from Holophaga/Acidobacterium or gram-positives) |
| 69b | 69 | Silt and clay | |
| δ | 211a | 212 | All fractions |
| Unclassified | 206 | 205 | All fractions (may also derive from Holophaga/Acidobacterium) |
| Planctomycetes | 230 | 231 | All fractions |
| Flexibacter/Cytophaga/ Bacteroides | 213a | 212 | All fractions |
| 235 | 236 | Silt and clay (may also derive from Holophaga/Acidobacterium and Prosthecobacter) |
a
Was detected in the T-RFLP profile of the AM clay fraction, but was lost during the normalization procedure.
b
Was not found in the T-RFLP profile of the AM clay fraction.
c
Was not detected in any T-RFLP profile.