Figure 1.

A) Synthesis of the new NAD⁺ analogs TMR‐NAD⁺ 1 and DTB‐NAD⁺ 2 starting from 2‐iodoadenosine (3) as precursor. TMP=trimethyl phosphate, DBU=1,8‐diazabicyclo[5.4.0]undec‐7‐ene, β‐NMN=β‐nicotin‐amide mononucleotide, CDI=N,N‐carbonyldiimidazole. B) SDS PAGE and western blot analysis of auto‐ADP‐ribosylation with ARTD1 using TMR‐NAD⁺ 1 or DTB‐NAD⁺ 2 thereby ARTD1 serves as its own acceptor. The total concentration of NAD⁺ in each reaction was 1 mm. The NAD⁺ derivatives were also tested in 1 : 1 ratio with natural NAD⁺ (lane 4 and 7). Controls were performed using either natural NAD⁺ (lane 2) or no enzyme (lane 5 and 8).