Figure 3.

Visualization of endogenous PAR formation using TMR‐NAD⁺ 1 and DTB‐NAD⁺ 2. HeLa cells were incubated with A) TMR‐NAD⁺ 1, DOTAP or B) DTB‐NAD⁺ 2, DOTAP transfection mixture, stressed with H₂O₂ as indicated and fixed. For the DTB‐NAD⁺ 2, the cells were transiently permeabilized and stained with Strep‐Cy5. Controls were performed without H₂O₂ treatment, with inhibitor Olaparib and without the transfection reagent DOTAP. Samples were analyzed by CLSM. Scale bar: 20 μm.