Figure 4.

Acceptance of DTB‐NAD⁺2 by different ARTD‐members investigated via an auto‐ADP‐ribosylation assay. SDS‐Page and western blot analysis of the respective member are shown. Reactions were performed using only DTB‐NAD⁺2 (lane 4, 5, 6) and a 1 : 1 ration of natural NAD⁺ and NAD‐DTB‐NAD⁺2. Controls were performed, without NAD⁺ and with natural. NAD⁺ (lane 1 and 2). The applied concentrations are indicated above the respective lanes. *No full‐length protein, mainly catalytic domain.