Table 1.
cDNA.
| Template RNA solution | 1ug |
|---|---|
| 5×Reverse Transcriptase Buffer | 4ul |
| dNTP Mixture(10 mM each) | 2ul |
| RNase Inhibitor | 20U |
| Oligo (dT) 18 Primer | 50 pmol |
| AMV Reverse Transcriptase | 10 U |
| DEPC H2O | up to 20ul |
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