FIGURE 4.

Ablation of Hmgb1 in Mst1/2 ^∆Hep mice decreases YAP⁺ nuclei, Tead mRNAs, and proliferation. Control, Hmgb1 ^∆Hep, Mst1/2 ^∆Hep, and Mst1/2&Hmgb1 ^∆Hep mice were generated and followed for the development of tumors for 2 or 3.5 months. (A) YAP IHC (yellow arrows show nuclear staining) (left) and quantification of the number of YAP⁺ cells in 10 fields at magnification ×200 (right) at 3.5 months (n = 3/group). (B) Relative liver mRNA levels of Tead1–4 at 2 months (n = 3–4/group). (C) Ki67 IHC (black arrows show Ki67⁺ nuclei) (left) at 3.5 months and quantification of the number of Ki67⁺ cells in 10 fields at magnification ×200 (right) (n = 3/group). Values are given as mean ± SEM. **p < 0.01, ***p < 0.001 for Mst1/2 ^∆Hep versus control. ^p < 0.05, ^^p < 0.01, ^^^p < 0.001 for Mst1/2&Hmgb1 ^ΔHep versus Mst1/2 ^∆Hep. Abbreviations: HMGB1, high‐mobility group box‐1; IHC, immunohistochemistry; mRNA, messenger RNA; MST1/2, mammalian sterile 20‐like 1 and 2; Tead, transcriptional enhancer factor domain; YAP, yes‐associated protein