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. 2022 Jul 12;13:921337. doi: 10.3389/fphar.2022.921337

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Copyright © 2022 Wang, Wang, Guo, Liao, Chai, Wang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Pre-study to detect efficient time points and concentrations. CCK-8 was used to evaluate cell viability to find efficient concentrations and key points. HUVECs were treated with LPS (0.25 μg/ml, 0.5 μg/ml, 1 μg/ml, 2 μg/ml) for 6 h (A), 12 h (B), and 24 h (C) to find efficient concentration and time point of LPS. HUVECs were stimulated with LPS (and RDN) and cultured for 6 h (D), 12 h (E), and 24 h (F) to find efficient concentration and time point of RDN. The RDN group was pretreated with cells diluted into different concentrations of RDN injection (dilution in 1/400, 1/200, 1/100, 1/50) for 1 h. Data are presented as mean ± SD (n = 3 per group) of the representative data from three independent experiments; **p < 0.01, ****p < 0.001, ^#### p < 0.001. The asterisk (*) represents the group is statistically different from the Con group.